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Old 08-02-2019, 04:17 AM   #1
HelenaSC
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Location: Spain

Join Date: Jun 2013
Posts: 21
Default Anyone using QIAGEN 16S/ITS Panel with MiSeq?

Hi there!

Anyone using QIAGEN 16S/ITS Panel with MiSeq?

We have good libraries with good profiles, quantified by TapeStation, Qubit and qPCR and we load them according to Qiagen instructions (dilute to 2nM and load at 10pM) and it result in very low cluster density...NaOH is working properly with all the other runs, the Miseq is also giving good results with other type of Runs....anyone is in the same situation? Or anyone is having good results with this Panel sequencing on a MiSeq?

Thanks a lot in advance!
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