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Old 08-02-2019, 07:37 AM   #2
kmcarr
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Location: USA, Midwest

Join Date: May 2008
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Are you sure that it really is low cluster density? Do you have access to the Thumbnails? When a flow cell is very overloaded it may falsely report a low cluster density. I ask because our experience with 16S/ITS amplicons on the MiSeq is that they need to be loaded at much lower concentrations (3.0pM). But this is with amplicon we make using the Schloss SOP. Amplicons prepared using a different protocol may require very different loading concentration.
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