View Single Post
Old 11-26-2019, 11:16 AM   #3
Tom2013
Member
 
Location: Canada

Join Date: Sep 2013
Posts: 19
Default

Quote:
Originally Posted by ATϟGC View Post
My guess is that you cannot use the "default" sequencing primers with your scheme.

You appear to have directly attached your gene-specific primer sequence directly to the i5 and i7 illumina indexing primers and therefore lack the sequencing primer binding site, which is actually contained within the Nextera transposase sequence.

Pages 3 and 4 of the following Illumina document might help you:

https://support.illumina.com/content...0002694-11.pdf
Thanks a lot for your reply.
Are there any document/post(s) mention that "the sequencing primer binding site is actually contained within the Nextera transposase sequence"?
Tom2013 is offline   Reply With Quote