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Old 07-28-2013, 03:37 AM   #22
Senior Member

Join Date: Jul 2013
Posts: 142

Thank Heisman
As I wrote earlier, I am almost a lone wolf :|
However, I have changed my plans.
Now want to detect clinically relevant Variants and might look something common between my my available RNA-seq data and thinking for 500 insert PE.
Can you please tell me your best strategies, if you were at my place ?
Thank you

Originally Posted by Heisman View Post
Sorry I did not respond earlier. I am confused. Why do you want to build your own reference genome? What do you mean in your last post when you say you are going for 500 reads? You seem to want to do a lot of different experiments; do you have people around you who you can further discuss this with?
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