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Old 12-20-2018, 04:02 AM   #3
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Location: East Coast USA

Join Date: Feb 2008
Posts: 6,888

MiSeq is a sequencing champ as far as illumina libraries are concerned. We look at results from MiSeq nano runs only qualitatively (rather than quantitatively) before the pools are transferred for final NovaSeq runs. You always need to keep in mind G = no signal limitation of 2-color chemistry as well.

If you are consistently observing that index combination performing badly then BioO would need to know that.
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