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Old 01-07-2019, 05:03 PM   #2
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Location: US

Join Date: Dec 2010
Posts: 372

Nothing about the read metrics raises warning flags - they could certainly have generated longer library molecules though since they did not multiplex the library.

Is the genome size about what you expected?

I would try additional assemblies with subsets of the data for examples only the longest reads. 50x coverage would be plenty. I would also try Canu as an alternative assembler.
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