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Old 03-31-2015, 05:23 PM   #2
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Location: East Coast USA

Join Date: Feb 2008
Posts: 7,049

When you are in investigative phase, dialing back on number of threads is a prudent thing. Start with a couple of threads and at least 24G of RAM. See if that job finishes. You would want to keep the threads on a physical server since having them going to different physical servers is not a good thing (I am not sure if you are already asking PBS Pro to do that, am an LSF/SGE user).

With TBLASTX I am not sure if 24G is going to be enough. Why not try a TBLASTN against a protein database? What are you trying to map (metagenomic data)?

Last edited by GenoMax; 03-31-2015 at 05:27 PM.
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