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Old 12-01-2015, 08:15 PM   #7
quokka
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Location: oz

Join Date: Apr 2010
Posts: 12
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Ok...it took me a long time before i was able to get back to this......

I essentially tried what tomc suggested. The problem I found was that the e-values were a bit different to what I was expecting....which complicates things when looking for a best blast hit....

I gather this discrepancy results from splitting up the database...which affects database size....which influences calculation of the e-value...

mpiblast is meant to overcome this problem....but i ran into a different problem with that ( http://seqanswers.com/forums/showthread.php?t=49325 )

......more tweaking is required!
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