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Old 03-13-2018, 12:02 PM   #1
GenoMax
Senior Member
 
Location: East Coast USA

Join Date: Feb 2008
Posts: 7,077
Default Core-facility NovaSeq users

I am interested in hearing from core-facilities that have NovaSeq's (or from other users where you don't always have control over library characteristics).

1. Do you pool libraries from multiple customers/sources? Do you insist on making the libraries or do you accept customer made libraries?
2. What QC measures do you use to ensure that you have a reasonably balanced starting pool/know what is actually in it (e.g. possible index submission errors from customers).
3. Do you run a MiSeq/iSeq diagnostic run before actual NovaSeq run?

Thanks in advance!
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