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Old 03-15-2018, 04:00 AM   #6
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Originally Posted by pmiguel View Post
(2) MiSeq titration run. We can tell from that if the customer was on the wrong index pair.

(3) Yes. Not a full run, just enough to allow titration. Alas the MiSeq run is not as good a predictive tool for relative cluster densities for the NovaSeq as it was for the HiSeq. Our Illumina FAS mentioned there was some hope that the iSeq might be better as it also has a patterned flowcell. But we don't have an iSeq, so I guess that is a moot point for us...

Hi Phillip,

Thank you for the useful information.

Can you clarify what do you mean by just enough to allow titration? You don't let the MiSeq run finish (I assume you are using 50x8x8 run)? You run the "pool of pools" that is going to be loaded on NovaSeq and not individual customer pools, correct?

AFAIK iSeq reagent costs are very similar to MiSeq so other than the possibility of having a platform with patterned wells it does not appear to have any major advantage. Illumina is also not discounting iSeq reagents for the foreseeable future.
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