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Old 03-15-2018, 08:10 AM   #7
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
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Quote:
Originally Posted by sbarberan View Post
Hi Phillip,

Really interesting points about purification.

You mention that using the RNA chip allows you to see primers/dimers even if they are annealed to full length library molecules; and that if this is the case you can purify them with AMPure.

My question is, if they are annealed to full length library molecules how come AMPure is able to remove them? AMPure will not denature the libraries so if they are annealed they will be co-purified, right? am I missing something?

Cheers,
sergio
Hi Sergio,
I agree. I would not expect it to work, but it does. We've tried a denature+snap cool prior to ampure and it performs the same as a non-pre-denatured ampure.
I have no explanation at the juncture, just giving the results we see.

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Phillip
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