View Single Post
Old 12-20-2018, 11:40 AM   #4
pmiguel
Senior Member
 
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,307
Default

You are running non-UDI indexes on the NovaSeq? Are you not concerned about index swapping?

We demultiplex allowing 0 mismatches for all Illumina runs these days. So I haven't taken note of non-concordant perfect index matches between the MiSeq and NovaSeq.

However, this is an issue mentioned in the Illumina White Paper on using an iSeq to titrate reactions for a NovaSeq.
--
Phillip
pmiguel is offline   Reply With Quote