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Old 01-25-2019, 06:15 AM   #10
ralyma
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Location: Copenhagen

Join Date: Aug 2018
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Dear Jakob,

We also have observed problems with H522=TTATGCGA. In demultiplexed data (default settings accepting 1-mismatch) 97% of observed adapters are TTTTGCGA.

To my knowledge TTTTGCGA is not an Illumina adaptor, so this makes it less likely that we have mixed H522 with another adaptor. Then it is more likely that there has been has been an issue in the production of H522 so that sequence TTTTGCGA has been produced instead of TTATGCGA.
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