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troendle · 10-31-2013, 08:29 AM

Hi everyone. I'm working on a small fish genome and want to know if anyone is having any success using the new fast flow cells for HiSeq that give >100bp reads as input along with a 3000bp mate pair read as the input for allpaths rather than making the 180bp library. The fast flow cells generate single reads rather than paired reads which is why I'm not sure if this will work. Any input is appreciated especially if you have tried this.

10-31-2013, 08:29 AM	#1
troendle Junior Member Location: Athens, Ga Join Date: Oct 2013 Posts: 2	Fast flow cells and Allpaths Hi everyone. I'm working on a small fish genome and want to know if anyone is having any success using the new fast flow cells for HiSeq that give >100bp reads as input along with a 3000bp mate pair read as the input for allpaths rather than making the 180bp library. The fast flow cells generate single reads rather than paired reads which is why I'm not sure if this will work. Any input is appreciated especially if you have tried this.