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Old 12-23-2016, 01:25 AM   #1
jrb71
Junior Member
 
Location: UK

Join Date: Dec 2014
Posts: 2
Default Alternative to NEB Blunt/TA ligase for ONT Rapid Sequencing Kit

Has anyone tried using a different TA ligase for library preparation? We've got a ton of KAPA Hyper Prep reagents (KK8503) which as far as I can work out can like for like substitute for the NEB Blunt/TA ligase master mix (and the NEB End repair/dA-tailing master mix though this isn't needed in the rapid library preparation). It seems a waste of cash to buy in new enzymes which we already have by the bucket load!
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