View Single Post
Old 12-23-2016, 01:30 AM   #2
David Eccles (gringer)
Location: Wellington, New Zealand

Join Date: May 2011
Posts: 843

Yes, I ran a flow cell in someone else's lab using some random other ligase that they had lying round. They just mixed the ligase with a buffer in the required concentration, and it was loaded as normal. The run worked, although I didn't have any comparable TA/ligase runs to benchmark against.

With the tiny amount required, you probably don't need the buffer, and could possibly get away with just adding a little bit more of anything that is ligase-like.
gringer is offline   Reply With Quote