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Old 10-06-2021, 05:22 AM   #1
hathormaat
Junior Member
 
Location: USA

Join Date: Jul 2021
Posts: 5
Default MinIon Prep Kit Selection (Ligation vs. Rapid)

Hey all,

I'm looking to sequence a handful of pure bacterial isolates for WGS and de novo assembly. Our group has decided to go with a combined Illumina/Oxford nanopore approach. While our Illumina protocols are well developed (MiSeq v3 using the Pulsenet protocol), our MinIon sequencing is something we're having to start (more or less) from the ground up. This would be an ongoing project sequencing around 2-12 isolates a month.

Does anyone have insight into which kits have worked best for them? A lot of the literature I see discusses using the Rapid Sequencing Kit (SQK-RAD004), but not much in the way of the Ligation Sequencing Kit (SQK-LSK109). For my purposes, will the Rapid sequencing kit (in combination with Illumina sequencing) be enough? Is the Ligation kit + Illumina effectively overkill? I don't want to suggest a kit that requires $1500 of reagents just for a $600 kit to get essentially the same results.

Thanks!

Last edited by hathormaat; 10-06-2021 at 10:39 AM.
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