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Old 12-03-2010, 12:26 PM   #2
mnkyboy
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Location: Seattle, WA

Join Date: Mar 2009
Posts: 87
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Use flip cap strip tubes this decreases the time getting them open and thus reducing the chance you lose your Cot curve.

Keep everything close and use two pipettors at a time (one in each hand), one to add the buffer or enzyme and the other to mix. This will reduce the time you have the lid off. You really want to move fast but do not want to introduce bubbles.

I think the protocol says to mix with a pipettor set at 40 ul but I do 35 ul since the 40 always introduces bubbles for me and if you remove the tube from the thermal cycler its game over.
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