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Old 03-08-2017, 06:27 AM   #16
SDPA_Pet
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Location: US

Join Date: Apr 2013
Posts: 222
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Quote:
Originally Posted by Markiyan View Post
Clustering means bridge amplification for pre ExAmp (non-patterned flowcells) - in situ PCR on the flow cell surface oligos lawn. Has similar rukes/laws to a regular PCR, only the product stays in situ, forming a forest from DNA strands.

For ExAmp Chemistry (patterned flowcells) - Clustering means cluster formation using Isothermal Amplification.
(In theory only on the occupied nanowell, in practice, especially at low loading concentrations a few neighbours may join in too...).

Have a read about ExAmp & Hiseq4000:
http://core-genomics.blogspot.co.uk/...d-to-know.html
Thank you. I did my sequencing on old platform HiSeq 2500.
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