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Old 03-10-2017, 05:15 PM   #2
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Location: Eugene, OR

Join Date: May 2013
Posts: 521

For RAD-Seq work in plants, PstI acts as if it is methylation-sensitive, as you'll get a number of cut sites sequenced that is consistent with the genome being several hundred megabases in size, regardless of the actual genome size (plants have a small amount of non-repetitive DNA with most of the variation in size coming from methylated repeats).

PstI cut sites tend to scale with genome size in mammals, so it acts insensitive there. These are not direct tests of sensitivity, of course, but it is consistent with the enzyme companies sites. So you are correct, I'd say!
Providing nextRAD genotyping and PacBio sequencing services.
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