Dear All,
this is my first post.
I have to make purchasing decision for a sequencer for the my lab in the next two months.
My need is simple - replace microarray gene expression profiling for formalin fixed paraffin embedded samples with DGE to get more reproducible data.
I looked at most recent protocols using DSN from Illuimina and SAGE-Seq from ABI. Illumina protcol looks simpler but new ABI 5500 seems to be very flexible since it allows sequencing in each lane and may be helpful for us to experiment with different conditions because we have to deal with samples from many different institutions.
Any recommendations?
thanks!
Soon Paik
NSABP
this is my first post.
I have to make purchasing decision for a sequencer for the my lab in the next two months.
My need is simple - replace microarray gene expression profiling for formalin fixed paraffin embedded samples with DGE to get more reproducible data.
I looked at most recent protocols using DSN from Illuimina and SAGE-Seq from ABI. Illumina protcol looks simpler but new ABI 5500 seems to be very flexible since it allows sequencing in each lane and may be helpful for us to experiment with different conditions because we have to deal with samples from many different institutions.
Any recommendations?
thanks!
Soon Paik
NSABP
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