I just got smallRNA-seq reads prepared by the TruSeq small RNA protocol from Illumina with index 1-7 and I am wondering what filtration is necessary?
I have started by removing the revers complement of the following PCR primer:
RNA PCR Primer, Index 1 (RPI1)
CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
Do I need to remove anything in addition, like any of these:
RNA 5’ Adapter (RA5), part # 15013205
5’ GUUCAGAGUUCUACAGUCCGACGAUC
RNA 3’ Adapter (RA3), part # 15013207
5’ TGGAATTCTCGGGTGCCAAGG
I have started by removing the revers complement of the following PCR primer:
RNA PCR Primer, Index 1 (RPI1)
CAAGCAGAAGACGGCATACGAGATCGTGATGTGACTGGAGTTCCTTGGCACCCGAGAATTCCA
Do I need to remove anything in addition, like any of these:
RNA 5’ Adapter (RA5), part # 15013205
5’ GUUCAGAGUUCUACAGUCCGACGAUC
RNA 3’ Adapter (RA3), part # 15013207
5’ TGGAATTCTCGGGTGCCAAGG