Hi,
I'm trying to assemble 454 raw reads (gDNA) with Newbler 2.6. Can anyone tell me what is the maximum volume of raw data (nt) newbler could intake in one-step or incremental form (as it could assemble large genome of up to 3Gb in size) ? Also the proportion of shotgun and mate-paired reads we should use in order to have a better assembly ?
I'm trying to assemble 454 raw reads (gDNA) with Newbler 2.6. Can anyone tell me what is the maximum volume of raw data (nt) newbler could intake in one-step or incremental form (as it could assemble large genome of up to 3Gb in size) ? Also the proportion of shotgun and mate-paired reads we should use in order to have a better assembly ?
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