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Old 06-14-2015, 10:40 AM   #19
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Location: London, UK

Join Date: Feb 2015
Posts: 5

Originally Posted by kobeho24 View Post
Up till now, I've already had seveal attempts. Nothing's wrong except for the activity. And now I am kinda puzzled. May I ask where did you get the pTXB1-Tn5 plasmid? Is it from Addgene?
I received the plasmid from Addgene, indeed. May I ask what equipment you use for the purification - i.e. AKTA etc? Have you tried both on-column and in solution assembly?
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