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Old 06-14-2015, 06:14 PM   #20
Location: HKUST, Hong Kong

Join Date: Apr 2015
Posts: 32

Originally Posted by daniel007 View Post
I received the plasmid from Addgene, indeed. May I ask what equipment you use for the purification - i.e. AKTA etc? Have you tried both on-column and in solution assembly?
I didn't use a specific equipment for protein purification. Bascially, I just followed every single step of the Genome Research paper. Moreover, I haven't tried on-column assembly yet, cuz I used to do the assembly in solution with a commersial Tn5 transposase, and it was not any issue.
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