I'm using Nextera to tagment full length cDNA from a heterogeneous mixture. Within this mix, there should also be a small dsDNA fragment that is 113 bp long. I do not want the Tn5 to do anything to this small fragment. I don't need it tagmented. My plan is to separate this 113 bp fragment from the longer tagmented cDNAs by Ampure XP, and take the supernatant (containing the small fragment).
The Nextera manual says Tn5 doesn't work on amplicons < 300 bp, but I'm not sure about that. does anyone have any thoughts on this?
Thanks.
The Nextera manual says Tn5 doesn't work on amplicons < 300 bp, but I'm not sure about that. does anyone have any thoughts on this?
Thanks.
Comment