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Old 07-13-2016, 03:43 PM   #7
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,174
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For these libraries double SPRI is not required but PCR need to be cleaned before sequencing anyway, so you can do 1x or 0.8x bead clean up (depending on the ratio used for clean up of fragmented input DNA). This will have the same size cut off as input and should not change the size distribution.
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