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Old 04-17-2018, 10:53 AM   #8
thermophile
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Location: CT

Join Date: Apr 2015
Posts: 233
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Phillip-what conditions have you seen primers annealed to full length amplicons? do you routinely heat denature then snap cool before cleaning amplicons?

OP- I look at %base to see what is happening in my amplicon runs. Does it look like the %base from your previous runs (all of which will be way out of normal Illumina spec)
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Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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