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Old 04-19-2018, 08:42 AM   #14
thermophile
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Location: CT

Join Date: Apr 2015
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Quote:
Originally Posted by pmiguel View Post
Yes, we tried it, although not extensively. It worked, just no better than without denaturation.

Remember there is nothing to stop the denatured molecules from re-annealing--especially at the adapter ends unless you include a denaturation.

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Phillip
good to know, thanks
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Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.
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