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Old 07-08-2013, 12:26 AM   #5
Location: Spain

Join Date: Jun 2013
Posts: 21

After reading all these posts, I wonder, can't I just use 0.1N NaOH (instead of 0.2N) to denature my libraries ,with no further neutralization step?
The issue is that I don't want to dilute my samples as much, so , maybe I can start with lower concentration of NaOH??

Thanks a lot!
HelenaSC is offline   Reply With Quote