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Old 07-18-2013, 07:20 AM   #12
Calluna
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Location: Seattle, WA

Join Date: Feb 2012
Posts: 12
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I'm glad your Q scores and PF improved. The first thing that comes to mind is to ask how you determined concentration of your libraries. If it was based off High Sensitivity BA, I get quite a bit of variability in accuracy. If you haven't already, I would suggest trying a qPCR to quant the libraries. Second, I would probably suggest giving Illumina tech support a call to see if they can go through your run data with you.

As far as your other thread goes http://seqanswers.com/forums/showthread.php?t=31491,
I'm not entirely sure what the extra hump would be from. It doesn't look broad enough to be bead carryover...maybe it is a bubble product, although your BA looks more like a shoulder than a bubble to me http://seqanswers.com/forums/showthr...ghlight=bubble.

Maybe someone else may have some insight.
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