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Old 07-18-2013, 07:20 AM   #12
Location: Seattle, WA

Join Date: Feb 2012
Posts: 12

I'm glad your Q scores and PF improved. The first thing that comes to mind is to ask how you determined concentration of your libraries. If it was based off High Sensitivity BA, I get quite a bit of variability in accuracy. If you haven't already, I would suggest trying a qPCR to quant the libraries. Second, I would probably suggest giving Illumina tech support a call to see if they can go through your run data with you.

As far as your other thread goes,
I'm not entirely sure what the extra hump would be from. It doesn't look broad enough to be bead carryover...maybe it is a bubble product, although your BA looks more like a shoulder than a bubble to me

Maybe someone else may have some insight.
Calluna is offline   Reply With Quote