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Old 07-18-2013, 07:28 AM   #13
HelenaSC
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Location: Spain

Join Date: Jun 2013
Posts: 21
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Hi again,

We quantify libraries using qPCR (KAPA Kit) and correcting by size with Bioanalyzer profile. Having a look at the results it seems that Chip is more "represented" than Input, and we think that the reason is the "strange " profile of chip samples (the correction by its size on bioanalyzer may not be ok, since the profile is more difuse or wide). So , maybe we should try these 2 things:

a) Correct by size again, in orde to have a more equitative pool.
b) Go for 16-17pM final concentration instead of 11pM and see if cluster density grows up, since the run seems to be "perfect" regarding the other metrics.

Thanks for your comments
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