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Old 07-18-2013, 07:28 AM   #13
Location: Spain

Join Date: Jun 2013
Posts: 21

Hi again,

We quantify libraries using qPCR (KAPA Kit) and correcting by size with Bioanalyzer profile. Having a look at the results it seems that Chip is more "represented" than Input, and we think that the reason is the "strange " profile of chip samples (the correction by its size on bioanalyzer may not be ok, since the profile is more difuse or wide). So , maybe we should try these 2 things:

a) Correct by size again, in orde to have a more equitative pool.
b) Go for 16-17pM final concentration instead of 11pM and see if cluster density grows up, since the run seems to be "perfect" regarding the other metrics.

Thanks for your comments
HelenaSC is offline   Reply With Quote