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Old 07-23-2015, 09:59 AM   #3
ptery
Junior Member
 
Location: San Diego

Join Date: Apr 2014
Posts: 3
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I use 70ul @ 100pM for the old IC kit. For Hi-Q kit, I first followed the protocol with 25ul @ 50pM with only 49% loading. So I increased loading to 25ul @ 200pM, the result is still not good. Finally I tried 25ul @ 400pM, none of those conditions give me good yield. (45M/chip at the most for 400pM loading).
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