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Old 07-22-2016, 10:57 AM   #1
kgoglin
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Location: San Diego

Join Date: Dec 2014
Posts: 17
Default Strange double-peak in Agilent RNA profile

We are learning to pay attention to extraneous peaks seen in RNA profiles from the Agilent RNA nano chip. If we see any sort of tiny peaks around the 23S peak, we automatically assume DNA contamination and repeat a second DNAse treatment. This seems to clear most of those tiny peaks and samples behave much better in downstream processing.

The last set of samples we extracted RNA from had to go through this second round of DNAse treatment but still a strange "double-peak" remained just before the 23S peak. Has anyone seen something like this before?

These particular RNAs were extracted with Trizol and glass beads were added to break up the cells. Perhaps the glass beads caused this somehow?
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