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Old 07-27-2016, 08:54 AM   #2
buthercup_ch
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Location: Japan

Join Date: Apr 2014
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Sorry but this amples are supposed to be total RNA, right?
Then why observing 2 peaks is strange? you should observe several peaks actually, mainly those for 16S and 23S if working with bacteria (as I assume you are doing), additionally another peak for 5S and small rRNAs and tRNAs and in some cases even an additional low intense peak higher at high molecular weight.
How is that possible that the peaks, that show the presence of undegraded RNA will disappear by treatment with DNAse?
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