View Single Post
Old 10-04-2011, 03:37 AM   #2
Senior Member
Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
Posts: 2,317

Hard to say without see an image. This could be the normal "bird-nesting/bubble product/daisy chain" hybe phenomenon one will often see with TruSeq libraries after the enrichment amplification. You could try denaturing aliquots from the library (95 oC for 2 minutes followed by "snap" cool on ice) prior to running them on a pico RNA chip. That should remove the inter-molecule hybridization that can produce the multiple/broad peaks and give you an additional a better idea of the true size range of your amplicons.

pmiguel is offline   Reply With Quote