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Old 10-04-2011, 03:37 AM   #2
pmiguel
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Location: Purdue University, West Lafayette, Indiana

Join Date: Aug 2008
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Hard to say without see an image. This could be the normal "bird-nesting/bubble product/daisy chain" hybe phenomenon one will often see with TruSeq libraries after the enrichment amplification. You could try denaturing aliquots from the library (95 oC for 2 minutes followed by "snap" cool on ice) prior to running them on a pico RNA chip. That should remove the inter-molecule hybridization that can produce the multiple/broad peaks and give you an additional a better idea of the true size range of your amplicons.

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Phillip
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