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Old 09-16-2016, 09:19 AM   #4
gconcepcion
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Location: Menlo Park

Join Date: Dec 2010
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This is characteristic of a loading issue as rhall indicated.

Unfortunately the ReadsOfInsert protocols don't give you the loading report information, however you can lump all of the cells together in a job (or do them separately) and map them to a reference using the RS_Resequencing workflow. One of the reports generated is a loading efficiency report that should give you an indication of how well the SMRTCell was loaded relative to the other ones.

You can use any reference if you don't care about the mapping and only want the loading report.

Attached is a sample loading report :
productivity 0 - means empty ZMWs
productivity 1 - ZMWs loaded with a single polymerase (what you want)
productivity 2 - ZMWs loaded with more than one polymerase (mostly unusable)
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File Type: png Screen Shot 2016-09-16 at 10.17.21 AM.png (109.3 KB, 7 views)
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