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Old 04-01-2019, 10:04 AM   #3
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Location: New Orleans, LA

Join Date: Apr 2019
Posts: 4

Thanks for the quick reply! But I'm still not sure I understand.

My thought is that the because the primer matched the DNA fragment during PCR, it can be considered a legitimate copy of a biological sequence of nucleotides, just like the copy that is made during sequencing.

But it seems from what you wrote that the problem is due to over representation of those sequences. How is a primer sequence different from a highly conserved region of an amplicon?

To be clear, I'm coming at this from a metagenomics perspective, which may be skewing my thoughts.

Last edited by sformel; 04-01-2019 at 10:06 AM. Reason: picture was too large
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