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Old 04-01-2019, 11:22 AM   #6
cmbetts
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Location: Bay Area

Join Date: Jun 2012
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That sequence isn't derived from the fragment you're trying to sequence. 100% of it was chemically synthesized by your oligo synthesis company. It match close enough to your insert of interest to prime it, but does not guarantee that the sequences were perfectly complementary. For instance, any mispriming artifacts will perfectly match your sequence of interest over the length of the primer...
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