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Old 01-23-2014, 09:11 AM   #6
Mike2188
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Location: Winnipeg

Join Date: Oct 2013
Posts: 26
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Some people have sequenced overamplified libraries with success if redoing is expensive or not much of an option. Also, people have reannealed their libraries. What happens is the DNA forms ds products that aren't complementary near the middle so they run funny on the bioanalyzer or gels, but they still have relevant sequencing information.

Best of luck with the rest of your experiment!
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