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  • MiSeq v3 600 Run Stopped at 301 cycle

    The process cannot access the file 'D:\Illumina\MiSeqTemp\170210_M03340_0029_000000000-ATAVH\Recipe\RunState.xml' because it is being used by another process. (System.IO.IOException)

    I was perfect loading cluster density of 1280
    Attached Files
    Last edited by rahulrahi24; 02-11-2017, 07:31 PM. Reason: Image upload

  • #2
    Have you contacted Illumina tech support? If they determine that this was a computer issue you would at least get replacement reagents for free.

    Comment


    • #3
      IO.Exception-> Check the SMART's or your's HDD.

      Contact the tech support, and

      It looks like HDD problem to me.

      Check the smart statuses of your MiSeq's HDD,
      especially Reallocated Sectors Count and
      Current Pending Sectors.

      If any of then >0, than replace the HDD.
      Last edited by Markiyan; 02-13-2017, 03:08 AM. Reason: Typo fix.

      Comment


      • #4
        Thanks

        Thanks Markiyan and GenoMax,

        Illumina Tech Support said it might be because of Windows and Illumina software issues. They asked to run a system check and power cycle the instrument. They are replacing the kit.

        I will check the SMARTs on HDD.


        Thanks

        Comment

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