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Old 02-14-2011, 10:19 PM   #1
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Location: us

Join Date: Feb 2011
Posts: 2
Default BLAST database error - when changing to new BLAST+ local program

I used local BLAST a while ago and have problem when changing to the new BLAST+.
I put the .../ncbi-blast-2.2.24+/bin and .../ncbi-blast-2.2.24+/db into $PATH

and execute the command
/local/.../blastp -query input -out output.fa -db nr -evalue 0.001

error returned:
BLAST Database error: No alias or index file found for protein database [nr] in search path [/local/.../ncbi-blast-2.2.24+/bin::]

What does this error mean?
Has the query input query format changes in the new BLAST+?
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