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Old 04-05-2019, 01:41 PM   #2
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Location: Bay Area

Join Date: Jun 2012
Posts: 121

Take a page out of old school RACE (or Tang/Quartz scRNA-Seq for a more modern example). Add a homopolymer tail using TdT, perform second strand with a complementary primer also with a defined tag, and then amplify with primers complementary to the tags. If using very low inputs, you may need to use semi-suppressive PCR to keep down the artifacts.
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