Does anyone understand how the Nextera Read1 and Read2 primers can work when they both have identical 19 bp regions at the 3' ends?
I would never think about designing a Sanger sequencing primer with that structure, nor a PCR primer. It's hard to believe these primers are possible with normal base composition.
The hinge sequence on Truseq primers has a similar issue, but with "only" 13 bp of identical sequence.
I would never think about designing a Sanger sequencing primer with that structure, nor a PCR primer. It's hard to believe these primers are possible with normal base composition.
The hinge sequence on Truseq primers has a similar issue, but with "only" 13 bp of identical sequence.
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