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Old 07-08-2013, 03:36 AM   #1
Simone78
Senior Member
 
Location: Basel (Switzerland)

Join Date: Oct 2010
Posts: 208
Default Nextera vs Nextera XT: whatīs the difference?

Hi all,
I tried to figure out the difference between Nextera and Nextera XT kit (for low input). With the Nextera kit I was never able to get good libraries when starting from <1 ng input DNA, while with the Nextera XT kit I got great results from <100 pg DNA.
I believe the enzyme is the same (I donīt think Illumina took the effort to engineer the transposase twice when they bought Epicentre Technologies...), so the difference must lie in the buffer.
I am quite sure that the Nextera kit uses a regular Tris+MgCl2 buffer, but what about the XT kit?
Any help is greatly appreciated!
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