SEQanswers - View Single Post - Nextera vs Nextera XT: what´s the difference?

Simone78 · 07-08-2013, 03:36 AM

Hi all,
I tried to figure out the difference between Nextera and Nextera XT kit (for low input). With the Nextera kit I was never able to get good libraries when starting from <1 ng input DNA, while with the Nextera XT kit I got great results from <100 pg DNA.
I believe the enzyme is the same (I don´t think Illumina took the effort to engineer the transposase twice when they bought Epicentre Technologies...), so the difference must lie in the buffer.
I am quite sure that the Nextera kit uses a regular Tris+MgCl2 buffer, but what about the XT kit?
Any help is greatly appreciated!

07-08-2013, 03:36 AM	#1
Simone78 Senior Member Location: Basel (Switzerland) Join Date: Oct 2010 Posts: 208	Nextera vs Nextera XT: what´s the difference? Hi all, I tried to figure out the difference between Nextera and Nextera XT kit (for low input). With the Nextera kit I was never able to get good libraries when starting from <1 ng input DNA, while with the Nextera XT kit I got great results from <100 pg DNA. I believe the enzyme is the same (I don´t think Illumina took the effort to engineer the transposase twice when they bought Epicentre Technologies...), so the difference must lie in the buffer. I am quite sure that the Nextera kit uses a regular Tris+MgCl2 buffer, but what about the XT kit? Any help is greatly appreciated!