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Old 12-01-2009, 10:32 AM   #1
smallcreek
Junior Member
 
Location: Florida

Join Date: Nov 2009
Posts: 4
Smile 25 bp/frag run vs 50 bp/frag run regarding N2S ratio and cost efficiency

Hi,
I am a newbie for Solid NGS. Our customers are mostly from academic professors who place orders to run a few of samples (2 samples most recently).
I realize that 25 bp/frag run can cut cost significantly because of two reasons:
1. the price per sample can be reduced 30%
2. when a customer needs to run only 2 samples, the whole flowcell run cost from the strip and buffer reduced 65%, assume the left 7 packages of the strip buffer will not expire before they are used for other customers.

I have two technical questions about 25 bp vs 50 bp.

1. It seems N2S ratio goes up after 25 bp cycles. Is that true that we get more matching beads/aligned reads if we do 25 bp run rather than 50 bp run?
2. It seems the total tag coverages between 25 bp and 50 bp are not too much different on a segmetn of a quad slide. Does 50 bp run have many other advantages over 25 bp run?

Thanks in advance,

smallcreek
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