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Old 05-12-2015, 12:40 AM   #13
kobeho24
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Location: HKUST, Hong Kong

Join Date: Apr 2015
Posts: 32
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Quote:
Originally Posted by Simone78 View Post
yes, we published it in Genome Research last July. Here´s the PMID: 25079858.
Best,
Simone
Hi Simone,
Recently I've got the plasmid from you guys as a generous gift, and gave a try to purify the Tn5 Tnp. After following every single step of the protocol (except for the competent strain C3013, we used BL21DE3 instead), there was no activity in the Tnp activity assay at all. The protein size is correct on the SDS PAGE Gel. So I guess there must be some issues in the transpososome assembly. Would you please give me some advice?

Best!
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