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Old 05-12-2015, 02:48 AM   #14
Simone78
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Location: Basel (Switzerland)

Join Date: Oct 2010
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Have you tried to do the assembly in solution as we describe in the paper? Just elute the tn5 from the column and add the oligos in equimolar conc with the Tn5 --> incubate 37 degrees --> ready to use. If that doesn´t work then you might have an issue with the Tn5.
Best,
Simone
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