SEQanswers - View Single Post - RNA-seq from single cells?

amcg · 11-26-2015, 08:34 AM

Hi all,
I'm hoping to use the Smart-seq2 protocol to make rna-seq libraries from small populations (200-500) of cells collected by FACS. In this case would you advise sorting them directly into the lysis buffer (triton-x and RNase inhibitor) described in the Nature Protocols paper, or would it be better to extract the RNA first and then proceed with the reverse transcription on purified RNA?

Many thanks!

11-26-2015, 08:34 AM	#167
amcg Junior Member Location: UK Join Date: Nov 2015 Posts: 1	Hi all, I'm hoping to use the Smart-seq2 protocol to make rna-seq libraries from small populations (200-500) of cells collected by FACS. In this case would you advise sorting them directly into the lysis buffer (triton-x and RNase inhibitor) described in the Nature Protocols paper, or would it be better to extract the RNA first and then proceed with the reverse transcription on purified RNA? Many thanks!