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Old 11-28-2015, 04:40 AM   #168
chaichontat
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Location: Bangkok

Join Date: Sep 2015
Posts: 3
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Hi Simone,

I used the Smart-seq2 protocol using the Superscript IV. For some reason, the reaction failed completely (no cDNA products were observed). When I did not add 1M betaine (Sigma) and 6mM MgCl2, the RT reaction went beautifully, but there's no template switching. The template was 10 ng of purified PBMCs RNA and the TSO was synthesized by Eurogentec. Could you provide me with the reaction conditions you used with SSRT IV?

Thanks!
Chaichontat
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